I use Fleischmann's rapid rise active yeast and sprinkle a few grains on the surface of my fly media. I dont refridgerate either my active or brewers yeast and have had great results from using yeast that is many months old. Not sure what the flies eat as they definately bulk up when transfered from a depleted culture to a new one. However I dont think they are intoxicated as the yeast would rather do aerobic respiration than anaerobic, but maybe in deep media there may be some ethanol production . :wink: