I had troubles once with some cultures, I dumped the cultures and all flys, bleached everything and started with fresh flys from a different vendor, I have never had the trouble repeat. This was when I first started using repashy and I think I was transferring unwanted bacteria from my homemade media. Repashy superfly has made life for me very simple, the cultures never crash, turn stinky, or anaerobic. I use a 3rd cup of dry media and add heated R.O. water untill it thickens like oatmeal, let it cool in the fridge, add flys and I'm done.