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Don't spray mite spray directly on or in you fly cultures.

Products like that are usually sprayed onto paper towels that the cultures are kept on. Alternatively you can buy mite paper by the roll and keep cultures on that. That helps prevents outside re-infestation. To deal with the mite population you already have in your cultures throw cultures out after a month, producing or not. You won't ever completely rid your cultures of mites, but you can drop them down to levels too low to notice.

In my experience, mites seem more prevalent in dry cultures. Whether that's because they actually prefer dry conditions or because older cultures happen to be drier, I don't know.
 
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