And here is a fresh culture awaiting flies(In about 2 secs )! The other is the producing culture. The downside of TP rolls is that they break down fast(with Hydei only, melanogaster do not take the same toll on the rolls). I will add banana peel scraps, and another piece of TP when it is becoming "logged".
Becareful adding banana peel scraps as they can have wild fruitfly eggs on them and it only takes one winged fly to develop in a culture to really screw it up.
If a few winged FFs get into a culture is it a problem? In one of my older cultures that at first wasn't sealed correctly, a few winged FFs got in, i've been trying my best to get rid of them during feeding, and my frog has eaten a few of them.
I believe it would be undesirable. The wingless/flightless phenotype is a recessive one, so if they mated with any of your cultured FFs you'd get a bunch of fliers.
Also, the wrong temperature will contribute to the birth of fliers in your cultures. I had a couple of cultures that had bunch of fliers due to warm temps
This I'm not too sure about. I believe that the mutations that cause the wingless and flightless mutations are deletion mutations, and if so a change in temperature would not rescue the phenotype. In this scenario I think that you might have gotten some sort of cross-contamination with the wild type flies.
Potential sources of infection include but are not limited to
1) a flier getting into the culture
2) a flier breeding through the lid or laying eggs into the culture through the lid
3) if fruit is used as a media additive, eggs or larva can get into the culture that way. ...
If you have fliers in the cultures and the cultures were not exposed to excessively high temperatures, then unless you can individually select the new flies for the next cultures you have to toss the culture as you won't be able to eliminate the fliers. Otherwise you have to deal with having flighted flies through the house.
Yes, it definitively sucks when you open a culture and flies fly out. When i got my fliers, i had them in my closet. I believed that the temp significantly rose;therefore, introducing fliers. I cant seem to have any other reason for my cultures to have fliers.
So yeah, it seems that the vestigial mutant is indeed temperature sensitive according to the link. I have wingless flies, and the temperature where they are cultured has risen above 81F several times, and this was not rescued the phenotype of the developing flies.
I'm still pretty sure that the wingless phenotype is caused by a change in the wg gene (although it might not be a deletion as I thought before). I have not found evidence that the wingless phenotype is rescued by temperature, even though the vestigial or flightless mutation is: Interactive Fly, Drosophila Interactive Fly, Drosophila
Unless you were able to pick out females that have not mated then you cannot be sure that you have removed the fliers..
You need to only use unmated females to start the next culture and allow them to only mate with wingless males..
how in the world do you do THAT??? You, and some of the other people on DB constantly amaze me with your depth of knowledge and experience...very intimidating...
This sounds just about as interesting to have to do as in making baby chicks into capon material....please don't tell me this is in any way practical...or someone just has too much time on their hands or is getting paid to do it...just really gets my brain going...
It is used to either demonstrate how different gene combinations work or to isolate out new mutations. Since the flies can retain sperm for different matings, you wouldn't be able to know how the cross worked out unless you could use unmated females.
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