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Discussion Starter · #1 ·
So, I just made my first fruit fly culture using joshs frogs fruit fly culture kit. I realized though that some of my fruit flies.. I guess drowned :( they are dead in the media. Is this usual? Is there a way to prevent this? Loop
 

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Discussion Starter · #3 ·
Honestly, I felt it was more in the middle of being liquidly and thick. Like I think it was average but that sounds pretty reasonable. Is there anyway that I can fix that and make the mixture more thicker. The culture was just made yesterday.
 

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Once the cups cool (as you should mix them with hot water) the media shouldn't move. I like to think of it like the DQ Blizzard (flip it upside down and it shouldn't drip down).
 

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Discussion Starter · #7 ·
Once the cups cool (as you should mix them with hot water) the media shouldn't move. I like to think of it like the DQ Blizzard (flip it upside down and it shouldn't drip down).
Hmm, perhaps I should use less water the next time then. It was slightly less thick than that.
 

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Did you follow the Josh's Frogs instructions? By follow, I mean measure and not eyeball. I've not had an issue with their recipe with their products.
 

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Discussion Starter · #9 ·
Yeah I used measuring cups. Perhaps I just underfilled. So, the drowning flies are unusual. I'll most likely end up remaking it, thankfully there are still plenty of live flies in the culture to transfer.
 

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I use the Josh's Frogs mixture. It's not unusual to have a few drowned flies in my experience. That's ok as long as the culture develops ok. Give it a few days and see what happens.
 

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Some may get stuck and that is normal . It should produce fine if you see flies . I lose a few or more every time and they pump out well. If you followed instructions and see flies I would just expect a booming culture shortly when it is to be expected. Try not to overthink it, and never hesitate to ask questions . That is what we are here for.
 

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Some flies stuck in the media is no big deal. You will work out the consistency you like with practice. Too thick, it dries out too quickly. Too thin, it is hard to get flies out with out everything slopping around.
 

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IMHO, regardless on the media. I poor 3/4 cup boiling DI water into 1/2 cup media. I do not stir, I let it sit overnight to cool to room temp. I then sprinkle a tiny(1/8 tsp) amount of cinnamon on top, twist excelsior into it. I then spread/pull the excelsior from the middle to the outer edges of the cups(limits the magots reaching the very top). This has worked for me for a few years now. I always have a few dead flies end up on the bottom. I have read all the mixing instructions but I always boil the DI water. This seems to make me the best batches. I will say josh's frogs media is very good! Best mod I have done is having a bed of Diotomatos earth in a tub. I put the cultures on that. I also run 3m double sided tape around the upper edge of the tub to catch mites. I have not had a collapsed culture yet. But i am vigilant with wiping the outside of the cultures down to remove any mits that find their way in!
OK long rant. Hope my 2 cents is helpful.
Eric
 

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You can easily create conditions in the culture cups conducive to drowning.

Warm wet media (even appropriately wet) causes condensation on the sides of the cups. I let my cultures cool and dry in the refrigerator with the lids off to help. The main reason I use the fridge is to shelter the open cups from wild flies. If I'm in a hurry I'll just wipe the insides with a paper towel. You can lose a lot of your seed flies to that initial condensation.

The pin-hole style lids that Josh's recommends don't ventilate as well as the fabric ones. This can also contribute to wet conditions.

You could be stuffing too much excelsior, which makes for a very wet bottom of culture.
 

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IMHO, regardless on the media. I poor 3/4 cup boiling DI water into 1/2 cup media. I do not stir, I let it sit overnight to cool to room temp. I then sprinkle a tiny(1/8 tsp) amount of cinnamon on top, twist excelsior into it. I then spread/pull the excelsior from the middle to the outer edges of the cups(limits the magots reaching the very top). This has worked for me for a few years now. I always have a few dead flies end up on the bottom. I have read all the mixing instructions but I always boil the DI water. This seems to make me the best batches. I will say josh's frogs media is very good! Best mod I have done is having a bed of Diotomatos earth in a tub. I put the cultures on that. I also run 3m double sided tape around the upper edge of the tub to catch mites. I have not had a collapsed culture yet. But i am vigilant with wiping the outside of the cultures down to remove any mits that find their way in!
OK long rant. Hope my 2 cents is helpful.
Eric
Fantastic post, total light bulb moment for me, never considered the double stick tape route, been using vaseline like a total noob.
 

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If I’m in a hurry, I won’t let the media solidify and cool. Doing so, I expect flies are going to get trapped in media.

Something I started doing is inserting the excelsior into the cooling wet media so that it solidifies into it. I imagine this provides a better avenue for emerging larvae.
 
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