[earthfrog]

What kind of flies are they, what temps are they kept at, do you see tiny white dots moving on the inside of the cup (etc. TBD)?

 

[earthfrog]

They are melos and I do see the little white dots you are talking about. They are kept at room temp (66-68 degrees F).

You'll want to find a way to get the temps up to about 80 for the flies to increase production. At those temps it will be at a crawl.

Also, the moving white dots are mites infesting your culture. If you got it shipped to you this way, get a refund/new one. If not, get a new one anyway---making new cultures from mite-infested ones doesn't pan out.

I always keep my cultures away from the frog tanks b/c of the mite issue, and I always feed from the newer cultures first and keep them away from the ones more than 7-10 days old. Keep surfaces clean and wiped down often, free of culture media/other things the mites could feed on.

 

[earthfrog]

Thanks for the info. What is the best way to combat mites?

The whole last pgh. I wrote just above.
Second best way is mite spray, but I would never allow it to get into the cultures. Some vendors have been saying to put it in the culture media, but that's not good for the frogs.