what FF cultures are you making?I would probably make a two cultures every week. This will probably be over kill but it is nice to know you always have enough. I make cultures every week and throw them out once they hit 4 weeks to avoid mites.... Right now I make 5 cultures a week for 3 tinc pairs, 4 groups of tinc froglets, 10 thumbnails, and about 10 thumbnail froglets. Hope this helps!