I was prone to making three or four cultures from one cup without adding flies from another cup, so over time the flies were inbreeding, , and I noticed a decrease in production, and the flies seemed to hatch out smaller. This took about 6 months too happen, and reversed it self when I seeded a culture using several diff. cups. I was curious if anybody else has done this? If you are having problems with production, when you were producing tons of flies a few months ago, then this may be your problem.