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No. Although some will say that they produce a little better when lit, many others have great production in the dark.
 

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I've been culturing in the dark for the past month and haven't noticed any difference since the change from ambient room light. This was melanos.
 

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I leave my cultures out on a shelf. They are exposed to only the light the sun shines in through my glass doors. My cultures continue to produce for 1.5 +/- months. After about a month I stop using the culture unless there is a real need for flies. In short, no artificial lighting needed, nor heat beyond room temp.

JBear
 

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I don't notice much difference due to lighting, however, when I keep mine in a incubator at 85F or so, they produce much faster.
 

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i keep mine in almost complete darkness. i find they produce better in the dark. one time i left a freshly made culture next to my frog rack and all the casings were on one side of the container ( away from the light ) at that time i decided to move all my cultures to complete darkness and they produce much better. but i do also get good results in standard room lighting where i use to keep them on a smaller shelving system next to one of my racks.
 

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i keep mine in almost complete darkness. i find they produce better in the dark. one time i left a freshly made culture next to my frog rack and all the casings were on one side of the container ( away from the light ) at that time i decided to move all my cultures to complete darkness and they produce much better. but i do also get good results in standard room lighting where i use to keep them on a smaller shelving system next to one of my racks.
I actually noticed this myself, a few times since I had to move my cultures to a cooler place (used to be in the same room as the heating system), but never considered light being the cause. But sure enough, checking some newer cultures, the side away from the light is the one covered in pupa
 

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According to Ed, fruit flies can produce retinol stored in their eyes from exposure to sunlight. Great source of Vitamin A which is a deficiency many frogs will have if not using the right vitamins.

I keep in a few different locations to avoid a mite crash. Some have very little light and others are totally dark.
 

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I go with needing light for vitamin a. Covering with a compete sealed lid and putting the culture in the dark for about 8 hrs. is a great way to draw up larva to harvest for feeding larva. Just an FYI how to get your frogs a nice treat.
 
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