So the first culture these fruit flies were very active. They would crawl up my feeding cup faster than most (I tap out a bunch into an empty 1qt container before putting them in the viv.) Second culture they were getting jumpy, third jumping more, hard to contain them in my prefeed cup. Forth a bunch are now flying. I think I need to start a new blood line... good thing I do more than 1 culture at a time, the others arent flying...yet.