Cultur media preference

1393 Views 7 Replies 5 Participants Last post by kyle1745, Aug 20, 2004

Discussion Starter · Aug 19, 2004

All froggers out there I would like to know your favorite ff media and why you like it so much?

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amphibianfreak

· #2 · Aug 19, 2004

I really like making my own, it is less expensive in the long run. Plus i can add more of or take out some of an ingredient to see if it yeilds more flies. I've also used the media Patrick Nabors sells, when i did use it i never had a problem with mites or mold or anything of that nature.

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Discussion Starter · #3 · Aug 19, 2004

I make my own and find it to be great. I also used Patrick Nabors media and it was great also.

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KeroKero

· #4 · Aug 19, 2004

Personal fav

I like ED's fly meat culture media and add a bunch of stuff to make my own 'enriched' version (peprika, sprulina and chlorella powder, also from ED's, been experimenting with other stuff). I've used carolina but had issues with it in the lab, but it was ok otherwise. As for home made... I've tried a couple (usually in emergency out of media situations) and now know anything made with potato flakes will reek and makes me sick.

People keep saying home made, but there are hundreds of recipes! Whats yours?!

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amphibianfreak

· #5 · Aug 19, 2004

I use the " Harvy Peterson Medium" from amphibiancare.com, but instead of adding 1/2 cup of water i add 1/4 cup water and 1/4 cup viniger ( still equals 1/2 cup). It works great for me and i have never had problems with the instant mashed potatoes having a bad smell.

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Michael Shrom

· #6 · Aug 20, 2004

I prefer Carolina Biological Supply formula 4-24. It smells a lot better than most of the other mixes. I occassionally cut it with potato flakes. I use Christines recipe if I run out of carolina.

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Dancing frogs

· #7 · Aug 20, 2004

I can't remember whose recipe it is but I use the "power mix". I'm sure you can find it if you do a search. I use it because its easy, effective, nutritious, doesn't stink too bad, fairly cheap, and I don't have to order, and wait for it.
I've also used Ed's, and it worked well, and is super easy to mix.

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kyle1745

· #8 · Aug 20, 2004

Great info here:
http://www.doylesdartden.com/fruitfly.html

I use this one, but I use 1:1 water vinegar rather than metyl paraben:

Home made "Carolina Mix" I now sell this mix. Click Here for more info.
by Christina Hanson
Normal Amount Large Batch
1 cup of powdered sugar
4 teaspoons Methyl paraben
8 cups potato flakes
1/2 - 1 cup of brewers yeast.

Makes approx. 30 1/3 cup cultures.
3 cups of powdered sugar
1/4 cup Methyl paraben
6 quarts cups potato flakes
1.5 - 3 cups of brewers yeast.

Makes approx. 2 gallons of media (approx 100 1/3 cup cultures).

Sift the powdered sugar and the Methyl paraben into a gallon or larger ziplock bag or container.
Shake well.

Add brewer yeast and shake again.

Add the potato flakes and brewers yeast to the ziplock bag and shake well.

Use the same amount as the Carolina Biological Mixture. I normally use 1/3 to 1/2 cups of media mixed with equal amounts of hot water in a quart jar. This is the media I most frequently use.
The cost is approx. $0.08 per culture

Note:
Methyl paraben, also called Tegosept is a mold inhibitor. It can be purchased from Carolina Biological 800-334-5551 / http://www.carolina.com. The following are the catalog numbers, amounts, and prices.
ba-87-6161 - 30g - $7.25
ba-87-6163 - 100g - $14.50
ba-87-6165 - 500g - $28.50

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