I'm jumping in late on this but large tanks are one of my favorite topics. I think there is much more to think about than numbers of frogs and some of this was discussed with sustainability. With a tank that large, you may have the opportunity to observe frogs at close to natural densities. If you you figure a square meter of territory per frog in the wild, then you would be looking at maybe 3 frogs in the tank. You could probably push that to 5 frogs and still have a fairly sustainable tank. If you stock the frogs at densities close to what is found in nature, then you have a really good chance of being able to produce enough plant biomass to support the insect biomass to support the frogs. Justin's suggestion of adding rotting fruit is a good one which would boost the tanks ability to produce food insects but I don't agree that you would have to sacrifice aesthetics. A tank that size could easily hide a few oranges or bananas without ever being visible. Also, you can always toss some leaf litter over the fruit which I think actually enhances the aesthetics because what you see is little insects crawling around in the leaf litter as you would in nature and you get to see much more natural feeding behaviors by the frogs.
As for specific recommendations, I would start with a single species and only add a few frogs. I would actually add no more frogs than you would put in a 20 gal. tank. You can always add more frogs later if you like but again, stocking the frogs at very low densities would give you the opportunity to observe things that are not possible in smaller tanks with higher densities. I personally don't have any problem with someone starting with a very large tank for learning dart frogs. IF you can produce the food volume you need to seed the tank, then you will find the volume of the tank very forgiving. As far as mixing species, you would probably be able to do it successfully in this size tank but I wouldn't do it right off the start. I think it's really important to learn each species you keep individually before mixing so you can more easily recognize signs of stress or abnormal behavior.
As far as seeding the tank, I would seed it with some good forest loam. That is going to provide the highest diversity of soil arthropods you could hope for. You might get a few undesirables like snails or millipedes but in a tank that size, I wouldn't worry about it.
If you do decide to mix species later on, it is important not to create hybrids as others have mentioned. Basically, you don't want to mix species within the following groups: tinc group (auratus, azureus, leucomelas, tinctorious, glactanotus?, castaneoticus?), Phyllobates group, histrionicus group (granuliferous, histrionicus, pumilio), and the quinquivittatus group (imitator, ventrimaculatus, reticulatus, fantasticus, intermedius, and the many similar species). That's a quick list off the top of my head so I'm sure I left many out.