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Old 01-24-2009, 07:58 PM
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Default sterilizing for mycobacterium

Is bleach and alcohol a good way to sterilize a tank if it had mycobacterium?
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Old 01-24-2009, 09:19 PM
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Default Re: sterilizing for mycobacterium

Alcohol is not.

With respect to bleach see http://www.hawaii.edu/ehso/bio/Hypochlorite.pdf

Note that you need to clean out all of the organic debris to be sure of disinfection as even a shallow film of organic in a corner will shield any spores in that location.

Ed
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Old 01-24-2009, 09:29 PM
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Default Re: sterilizing for mycobacterium

Hmm, I work with native american fish and we've been told that the only way to kill mycobacterium is with alcohol.

A quote from our handbook

Quote:
1.Associates should wipe hands thoroughly with alcohol after coming into contact with any aquarium water. Work surfaces in food preparation, veterinary, and necropsy areas as well infected aquariums or cages should be cleaned with a 1:100 solution of household bleach in water( this solution must be made fresh each day due to chemical breakdown), Hepacide Quatâ, or Roccal after each use. The surfaces should then be wiped with alcohol since only alcohol will kill Mycobacteria.
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Old 01-24-2009, 09:31 PM
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Default Re: sterilizing for mycobacterium

Hi Tim

Edit... I was incorrect. Apparently alcohol does work for Mycobaterium I had been led to believe that it didn't work well for the resting spores.


Ed

Last edited by Ed; 01-24-2009 at 09:59 PM.
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Old 01-25-2009, 12:07 AM
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Default Re: sterilizing for mycobacterium

Hey Ed, I don't know if your wrong. Usually you are right so I basically posted that up to get your opinion since it differed from the information I had.
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Old 01-25-2009, 12:29 AM
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Default Re: sterilizing for mycobacterium

This should help: Disinfection, Sterilization, and ... - Google Book Search
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Old 01-25-2009, 12:31 AM
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Default Re: sterilizing for mycobacterium

Basically, I'd like to sterilize some LECA and aquarium gravel. None of my animals have been diagnosed with mycobacterium, but I just want to take extra care since the gravel came from a silurana (xenopus) tank.
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Old 01-25-2009, 12:48 AM
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Default Re: sterilizing for mycobacterium

What about heat?
Quote:
Originally Posted by Rain_Frog View Post
Basically, I'd like to sterilize some LECA and aquarium gravel. None of my animals have been diagnosed with mycobacterium, but I just want to take extra care since the gravel came from a silurana (xenopus) tank.
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Old 01-25-2009, 01:25 AM
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Default Re: sterilizing for mycobacterium

supposedly, you shouldn't use hot water (though I have in the past) on aquarium gravel because of the epoxy coating i think.

Also, I'm not sure what temp myco spores will survive.
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Old 01-25-2009, 02:52 AM
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Red face Re: sterilizing for mycobacterium

Ok, I ran off and found my Microbiology text. It says that mycobacteria dont' form endospores- which is why alcohol should work just fine for killing them. Additionally, it does say that boiling temps will kill 'vegetative forms of bacteral pathogens, almost all viruses, and fungi and their spores within about 10 minutes, usually much faster.' I haven't heard much about viruses being a problem with our frogs, and endospores are pretty darn tough to kill... so it sounds like good 'ol boiling water may be a good bet- cheap, effective, and safe once it cools!
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Old 01-25-2009, 05:34 AM
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Default Re: sterilizing for mycobacterium

You could bake the LECA in an oven provided the temperature is sufficient to kill everything.
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Old 01-25-2009, 03:25 PM
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Default Re: sterilizing for mycobacterium

I have boiled LECA before with success, but the instructions for my gravel says not to do that. I have done it in the past to gravel, but guess it's not the best thing to do since they coat it with epoxy.

Ed, any thoughts?
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Old 01-25-2009, 10:43 PM
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Default Re: sterilizing for mycobacterium

The whole virus thing is still really new and research is still lacking for the most part. But if you want to get an idea look up rana viruses, tiger salamander viruses...

Ed
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Old 01-25-2009, 10:53 PM
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Default Re: sterilizing for mycobacterium

Quote:
Originally Posted by Rain_Frog View Post
I have boiled LECA before with success, but the instructions for my gravel says not to do that. I have done it in the past to gravel, but guess it's not the best thing to do since they coat it with epoxy.
Fish stores usually sell natural gravel (no epoxy) by the pound. Don't know if there are any near you, but it's a lot cheaper than "aquarium gravel". Sometimes Home Depot will stock large bags of 1/8"-1/4" stones as well.
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Old 01-26-2009, 04:27 PM
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Default Re: sterilizing for mycobacterium

Ed, what is your recommendation for killing mycobacterium spores?
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