Please direct yourself to the following thread to review what has already been documented for working methods:
The following will be my attempts at culturing TWO different aphids that I collected from my front yard.
Every year my Hansa rose gets clobbered with aphids, this season I decided to collect them as additional feeders. I looked into the aforementioned thread and into the advanced documents listed and want to run with one specific culturing method from: Continuous Rearing of the Pea Aphid
The paper so that everyone may benefit/follow along. I do not wish to review the paper in depth, simply points that need to be addressed.
I chose this method because I have 100mm wide Parafilm-M on hand [for other sciences] and thus familiar. The problem with the stated setup for the hobby is that is uses expensive custom glassware - and so I would like to build a comparable setup with plastic jars & funnels. One of the other papers listed mentioned the diet being similar to what is in yeast extract, and so I have the half thought of using diluted Vegemite (available to near everyone) as the only liquid nutrient source.
But first I need to establish some cultures of aphids before jumping onto something more advanced. So one week ago I soaked some sweet pea seeds for 24hrs, and then rolled them in paper towels and into a cup with water that gets wicked. I didn't exactly forget about them, but I was slow at transferring the sprouts into a cup of soil (gathered from my herb garden). I was also not in a rush because even after I got rid of the aphids on the rose, they would be back within a week. I picked today to collect because there will be a small amount of rain here tomorrow - though the bugs don't go anywhere.
This afternoon on my way to the mailbox I noticed a dandelion covered in a recognizably different aphid.. I had made two cups of pea sprouts a day or two ago - and so it was decided to collect both types. It is possible for the rose to have had more than one type of aphid, but I am sure one species will rule in a culture.
Using a paint brush (per NathalieB) I knocked off as many as I could from the rose plant into a small cup and transferred them all (~150?) to a pea cup. The same was done for the dandelion, but I was able to break it off and nearly just shake them off, maybe 300?.. certainly don't want to break my rose! .. plus the rose has thorns.
After a few simple usb microscope shots (my vintage Leitz Toolmaker's microscope does not have a camera in it, yet)..
I can presume the rose aphid is in fact Macrosiphum rosae - both the pink and green varieties were collected.
The dandelion based one may be of genus Metopolophium? Included is a top down and underside image.
That is really the progress thus far.. that today I did my first transfers of two different wild aphids to pea sprouts in separate jars - and I plan on continuing the pea sprout method for at least 2 more generations to have an established isolated culture - but the plan will be to switch over to liquid diet on a setup similar to the attached paper.
I hope to keep everyone updated as progress is made - and please suggest any thing that may help our efforts.