Originally Posted by FrogTim
Your problem is mites. I had the same issue. The solution includes multiple steps.
-make sure all deli cups, lids, excelsior or whatever your using are sterile. I microwave the deli cup and lid for 45seconds before adding the media(don't microwave the media)
-create a barrier for mites with mite paper or diatomaceous earth. I keep every week's cultures(2) in its own shoe box with a thin sprinkling of DE in between. Make sure the cultures aren't physically touching either to prevent mites spreading.
-start cultures with clean cultures. If a culture has mites, any seeded culture will have mites. Some have had success dusting flies w vitamin powder to remove the mites before seeding, but that didn't work for me. Best bet is to get clean new cultures.
-throw away cultures after 30 days! Even if they're producing, just do it. Old cultures are ground zero for mite infestations. I freeze all my old cultures just to make sure everything is dead.
Hope you get it sorted! There's nothing more stressful than constantly running out of food for your frogs. I had to switch to Turkish gliders after all my melos crashed due to mites. It happens
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I already follow all these steps, originally all of these cultures were started from clean cultures. But even with mite paper, they all developed mites. I read somewhere that the type of mite that infests our cultures are always present in our homes.