Originally Posted by Johanovich
I'll see if I can get this done somewhere in the next weeks, it might require some tweaking with cycle nr etc. and ideally I'll want a positive control for madagascariensis (but that's likely going to be difficult to get).
haha, yeah, there's still lots of old school genetics that gets done
The only real drawback for this approach is the fidelity of the available information. The paper is behind a paywall for me, so I'm not sure how rigorous their data collection methodology is. That said, the BLAST algorithm should
prevent non species-specific primer binding. The primer design past is based on Primer3, which is also pretty accurate and trusted.
In this case a real positive control would be hard to get ahold of, due to the lack of information. But a well-designed assay would go a long way. You could start with just one individual to test things out, and run the baroni/madagascariensis primer sets in different tubes to avoid potential complications. And then as long as you only really get an amplicon of the expected size, you should be good.
OH and I guess the other thing you'd need is some sort of kit for amplifying genomic DNA from swabs or other similar method.
But yeah, this would be a nice thing for us to have in the hobby, to confirm/prove the identities of baroni/madagascariensis. Other methods are completely subjective...