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Old 10-05-2019, 04:30 AM
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hypostatic hypostatic is offline
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Default Re: Mantella baroni introgression? Very picture heavy!

Quote:
Originally Posted by Johanovich View Post
I think you're gravely underestimating the ease and costs of DNA sequencing. Having a PCR machine in the lab (which we obviously do) does not help for sequencing on its own, you need a separate machine for that. There's also no need to sequence the whole genome, this is unnecessary as we can use some specific genes for species identification. Published data is available for these genes, so that would greatly help identification.
Well that's why I asked about whether reference genomes were available for the species. If they're published, you just need primers for stretches of DNA that are different for each species, and you can just do a regular genotyping PCR run. Sequencing wouldn't be necessary.

OH HEY, while the whole genome hasn't been published, it does look like there is some mtDNA data available:
https://www.ncbi.nlm.nih.gov/bioproject/?term=mantella

Here are specific primers based on that information:
Baroni
Madagascariensis

So yeah... you should be able to tell the species based on whether you get amplification or not, and by PCR band size...

Assuming you have access to everything else needed to run a PCR, the primers would only set you back about $40....
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