Re: sterilizing FF culture cups
I do not think infestations originate from containers. As illustrated from the posts shared, the varied protocols applied by posters differ in personal preference, but all include adequate heat. If coverage is complete, kill temp is a conventional 130 F. With mites it is instant. There are oocysts eg, that require higher temps for a lengthier duration, and "true" sterilization - the elimination of all microorganisms, autoclave, long dwell high heat, or agent contact, a common duration point is 10 min.
The environment, media materials and the transferred seeding flies themselves (hitchhiking inadvertently in hairs) are an open link.
It would be great if there was only one thing to control, and all it took was the perfect wash technique of the containers.