About 3 weeks ago I mentioned losing a little terrarium fern, Quercifilix zeylanica, and that I would try growing the spores that were left on dead fronds.
It's working.
Here are the containers and dried fronds on 11/13/09. These fronds just were left standing on dead plants since August, when they were killed by hot dry weather, until I finally cleaned out that terrarium in mid-November. For some reason they never made it into any pictures when they were alive.
You can see the spore packets rubbed off on paper are huge- the dried frond in the close-up is over an inch long. The spores stayed in these seed-like packets instead of coming apart into dust, so I just left them that way.
I planted them on Black Gold "Cocoblend" potting soil, screened through a kitchen sieve. I didn't "sterilize" the soil, but the bag was pretty new and was probably pasteurized at the factory. I washed my hands, rubbed the spore packets off the frond, sprinkled them lightly over the soil, and misted them a little bit. After sealing the containers I put them in the back of a terrarium where they would get bright but not direct light.
I ignored them for about 3 weeks because nothing ever grows when I watch it every day. Here's what I found just now:
You can (sort of) see that the prothalli are growing in tight little clusters from each spot where a spore packet fell. Next time I'll try crushing them to see if they won't spread out a little more evenly.
Temperature where these is just room temp, ranging from about 60°F at night to low-mid 70s days.
I just misted them lightly again to give the sperm some swimming room, just in case they're already releasing any. I'll update when anything new happens, but it might be a while before I see gametophyte fronds. I'm really hoping to see some variation when they mature, apparently wild types come in all kinds of shapes.
Vincent
It's working.
Here are the containers and dried fronds on 11/13/09. These fronds just were left standing on dead plants since August, when they were killed by hot dry weather, until I finally cleaned out that terrarium in mid-November. For some reason they never made it into any pictures when they were alive.
You can see the spore packets rubbed off on paper are huge- the dried frond in the close-up is over an inch long. The spores stayed in these seed-like packets instead of coming apart into dust, so I just left them that way.


I planted them on Black Gold "Cocoblend" potting soil, screened through a kitchen sieve. I didn't "sterilize" the soil, but the bag was pretty new and was probably pasteurized at the factory. I washed my hands, rubbed the spore packets off the frond, sprinkled them lightly over the soil, and misted them a little bit. After sealing the containers I put them in the back of a terrarium where they would get bright but not direct light.
I ignored them for about 3 weeks because nothing ever grows when I watch it every day. Here's what I found just now:



You can (sort of) see that the prothalli are growing in tight little clusters from each spot where a spore packet fell. Next time I'll try crushing them to see if they won't spread out a little more evenly.
Temperature where these is just room temp, ranging from about 60°F at night to low-mid 70s days.
I just misted them lightly again to give the sperm some swimming room, just in case they're already releasing any. I'll update when anything new happens, but it might be a while before I see gametophyte fronds. I'm really hoping to see some variation when they mature, apparently wild types come in all kinds of shapes.
Vincent