From your pic it seems the way to banish them is to return the one ring to the fires of mount doom from whence it came

 

We are going to start with the following supplies.
1) A clean, sterile culture container and sterile media. I am using a 24 cup Rubbermaid container because it seals very tightly and will help keep our new culture clean. Because it seals tightly, suffocation will eventually occur if we don't ventilate it. I use .3 micron filters, hot glued into the lid for a filter. You can get your .3 micron filter disks here Fungi Perfecti: micron air filters I use hot glue because silicone does not stick well to the rubbermaid container.
2) A large pan with high sides
3) A standard cereal bowl
4) 2 straws, taped end to end
5) rubbing alcohol
6) a couple paper towels
7) whatever you need for your chosen method of harvesting springtails

Thanks to Frogparty for the idea and link for the .3 micron filter disks.

 

Clean your tabletop thoroughly. Clean all your supplies thoroughly. A couple of mites on your supplies or on your table can ruin your efforts.
Fill your cereal bowl about 2/3 to 3/4 full of water. Harvest a large portion of springtails via your chosen method. Here, you can see how I sometimes harvest. I keep a piece a sterile tree fern panel on top of a coco fiber and peat, springtail culture. I hold the tree fern over the bowl of water and tap it rather aggressively with a spoon, or something. In the picture on the last post, you can see the pestle, from a mortar and pestle, that I sometimes use to tap it with. Thanks to James67 and Michael for sharing this method with us.

 

what is this un prepared nonsense!?! i am sitting here anxiously awaiting info.... come on doug get it together! its like christmas!

this is my mite cleaning device

happy friday guys!

 

Now I'm going to take a break for several hours just to spite Moty! Everybody thank him.

 

Here's my harvest. I want to point out the minimum of dirt in our cereal bowl. Dirt means mites and mite eggs. I also want to remind you that if you collected your springtails in your clean area, it is no longer clean. Get a paper towel wet with rubbing alcohol and clean the table again. This part is crucial to success! Clean the outside of the cereal bowl thoroughly with rubbing alcohol. I also carefully clean the inside lip of the cereal bowl. We have to make sure that any mites transferred are IN THE WATER and nowhere else!

 

Carefully set the cereal bowl into the large pan. If you spill a single drop of water you have to stop and clean up the large pan and the cereal bowl with rubbing alcohol again.

 

Take your straw and blow gently on your springtails. Blowing across at an angle is helpful. What we are doing here is separating the clean springtails from the mites. Springtails are masters of walking on water, while mites seem to get stuck in the top film of the water.
Remember, you want to blow as gently as possible to facilitate the transfer. If you blow hard enough to transfer any dirt at all, or even the tiniest bit of water, your efforts are in vain.

 

Now you see why we wanted a tall pot to do this in. If you don't have a big enough stew pot, a bucket would suffice.
Pull the cereal bowl out and set it aside. You can see why we had to be careful that the outside of the cereal bowl was mite-free. If it wasn't, you have already contaminated your culture before you even finished.
Here is a shot of my nice, fully cleaned, harvest of white temperate springtails.

 

Here I've tipped the pot to the side and tapped them down into one pile for you to see.
This is the harvest that I tapped out of ONE 4" x 4" tree fern panel square. Just throwing that in for anybody wondering how well the tree fern panel harvest works.

 

Dump them into your new, sterile culture and feed 100% active bakers yeast. I dumped 2 harvests into this culture so it is started with many thousands of springtails. I fully cleaned the bowl between harvests.
Using the tree fern harvest leaves plenty of springtails behind in the old cultures so I will probably do this a few more times, giving me a new, master culture in no time.
Of course you can continue to harvest out of your old cultures until your new one is producing.
Put your new culture AWAY from your old ones and on a piece of mite paper.

For further information on culturing springtails and isopods, please check out my culturing thread. http://www.dendroboard.com/forum/food-feeding/66991-how-culture-isopods-woodlice-springtails.html

As you may have guessed, I am gearing back up to supply you with some fresh, clean cultures. I want to be ready for when this horrid, cold, wintery crap goes away!

 

Next, let's take a quick look back at how I have cleaned large springtails (Giant Black Tomocerus) in the past. If it seems familiar, I've posted this method before but thought it should go here.
For a large springtail like a Tomocerus, here is what I do. Collect a small amount from your contaminated culture. This will obviously have to include some of the target critters, Tomocerus in this case. It can also include media and the contaminant (springtails, mites, or whatever). Put a lid on it and put it in the fridge for a while. It may take an hour or so before they get sluggish. Now take off the lid and put in the freezer for about 30 seconds to a minute. At this point they will look dead. Quickly pour them onto a refrigerated kitchen plate. You may want to get your wife/girlfriend out of the house for a while before pouring bugs all over her kitchen plates!! Refrigerating the plate gives you longer working time before they "wake up" and start jumping around. I also like to put a couple of refrigerated gel paks under the plate. Using a tiny modelers paintbrush, quickly seperate about 30 of the largest ones. Make sure there are no contaminants in your separated sample. Brush them into your new, sterilized, media. Feed em and close them up tight. You should make several small cultures in case you messed one up and got a few whites in the mix. As you are starting with a small sample, it will show up quickly if you messed one up. Good thing you made 3 or 4!
IMPORTANT Work with only a small portion of the master culture at once! If you get distracted and leave it in the fridge or freezer too long, they will die. You don't want to risk your entire culture.
Once they are producing well and you know they are clean, Dump your old contaminated culture into one of your vivs.
For obvious reasons, this method works best with large springtails like Tomocerus.

A second method I have done with Tomocerus is to harvest some onto a small, clean plate, and put it into my tall stew pot. Wait about one minute till some jump off the plate. If you wait too long, mites can crawl off the plate into your pan so you are only going to harvest a very small percentage this way. Dump your plate back into your old culture and dump the pot into your new, sterile culture. Clean the plate well and repeat.

 

How to clean your Isopod cultures

This is a little more time consuming. Put a small pile of flake fish food or whatever food your isopods can't get enough of on top of the culture. Give them overnight to be attracted to it.
Get a pint sized deli container or some other container and put a damp paper towel on the bottom.
Pull a handful of isopods and media out and put it on the center of a dinner plate.
Using a small modelers paintbrush you can carefully brush your isopods across to the edge of the plate where you will knock them off into the deli cup. This is going to take some time. Don't worry about the babies, just get as many adults as you feel like taking the time to get.
Dump the rest of the plate back into your old culture. You will continue to feed out of the old one until your new one is producing well.
Now put a lid on the deli cup and shake it up a bit. You are trying to knock loose any clinging mites or springtails off of the mites.
Dump the deli cup onto a CLEAN plate. You will have to use your CLEAN modelers paintbrush to knock them off of the paper towel and onto the plate.
Now prepare another CLEAN deli cup with a CLEAN, damp paper towel.
Repeat the process. Doing it twice like this just helps to remove any clinging mites or springtails.
When you are done, transfer the cleaned isopods into your new, sterile container. Don't forget to make sure it is tightly sealed and ventilated.

 

Doug,

Could you dust the Isopods when in the deli cup to help with removing the mites? Would this be a bad idea?

Bo

 

That sounds like it would be worth a trial run. Don't do them all at once because I really don't know if it would interfere with their breathing in any way.

 

Nice method. What about mites attached to the springtails?

 

I would think that whacking the tree fern panel during the initial harvest would knock them loose.

 

I pulled giant oranges from a mite infested culture and dusted them with Reptical before putting them in an new clean culture. Its been a few weeks so far and no signs of mites yet.

 

excellent write-up doug.

james

 

awesome ! Great method, thanks for posting ! hopefully I will never have to use it (knock on wood)

 

wow, this is cool, i may just do this for fun!

 

As an additional precaution I have begun taking the initial harvest (uncleaned) and dumping it through a funnel, into a Gatorade bottle half full of water. You can shake this up pretty good with no apparent harm to the springtails. The springtails pop right back up to the surface.
I pour this into the cereal bowl, set it in the pot, and blow the springtails off.
Clean and alcohol wipe the funnel and pour the cleaned springtail harvest into a second, clean, Gatorade bottle to repeat the process so they are double washed, and double blown off.

If anyone has any other other, possibly better methods, please feel free to post them here.

 

Update: The "float and blow" method I used on the Pinks has produced clean cultures with no signs of mites.
This method, A second method I have done with Tomocerus is to harvest some onto a small, clean plate, and put it into my tall stew pot. Wait about one minute till some jump off the plate. If you wait too long, mites can crawl off the plate into your pan so you are only going to harvest a very small percentage this way. Dump your plate back into your old culture and dump the pot into your new, sterile culture. Clean the plate well and repeat. was a complete failure.

 

I've cleaned a pink springtail culture that was CX with folsoma by picking up the charcoal pieces from the culture, shaking it a bit, and then blowing the pinks into the new culture. The folsoma seem much more ready to jump I've noticed (which is why they CX other cultures so easily), so most of them will jump off when you pick up a charcoal piece, and the rest will do it when you shake or tap it a bit. The pinks hold onto the substrate more readily, so most of them will still be on the charcoal, and they can be blown onto the new culture.

For best results the new culture should be set up some distance from the CX so the folsoma don't have a chance to jump into the new culture.

 

Summary

I created this summary for myself and thought "why not share it?"
Please read carefully through this entire thread in order to understand this summary. I hope Pumilo does not mind me summarizing his well written procedure.

Materials:
1) A 24 cup Rubbermaid container with .3 micron filters, hot glued into the lid.
2) A cereal bowl
3) An empty Gatorade bottle (or similar)
4) A large pan with high sides or bucket
5) 2 straws, taped end to end
6) Rubbing alcohol
7) Paper towels
8) Whatever you need for your chosen method of harvesting springtails

Procedure:
1) Clean your tabletop thoroughly. Clean all your supplies thoroughly.
2) Harvest a large portion of springtails via your chosen method.
3) Dump the harvest through a funnel into a Gatorade bottle half full of water.
4) Shake well! (no harm to the springtails, which pop right back up to the surface)
5) Pour this into a cereal bowl
6) Clean everything again, including all parts of the cereal bowl not occupied by water + springtails
7) Place cereal bowl inside the pot
8) Gently blow the springtails off on a 45 degrees angle
9) Clean the funnel and Gatorade bottle with boiling water
10) Repeat steps 3 to 8 (for extra safety)
11) Dump them into a new, sterile culture and feed 100% active baker’s yeast.
12) Put the new culture away from your old ones, preferably on mite paper.

 

I don't mind at all, nice summary. The only thing I would change is step number 9. I'm not sure a Gatorade bottle will survive boiling. I just use really hot tap water. It's only a "surface kill" we need, there is nothing it has to penetrate.

 

and also, make sure you have a pot/bucket that is at least 18" tall because i tried it with my 12" pot and ended up losing half my springs cuz they all jumped out...

 

The pot I use is only 9.5 inches tall and I lose virtually zero springtails in the process. The only ones lost are still left in the cereal bowl, because if you blow hard enough to get every last one, you may be blowing hard enough to lose some water. Mites could be in a single droplet. I use a cereal bowl to hold the water and springs while I GENTLY blow them off. If you have to blow that hard, you are doing something wrong. Is your cereal bowl almost completely filled with water? If not, I can see how you would have to blow that hard.

 

So this all makes perfect sense to me, and seems to be a very effective way of ridding your culture of mites. But in the beginning you said that mites will get in almost inevitably anyways. So is this a temporary solution? Or is there a method in which to prevent the problem?

I generally do not stack my cultures, line the container or shelving with felt sprayed with either prevent o mite, jurraso mite, and some other store bought pet safe mite killer. Somehow they still get in no matter how careful I am. Does this mean we just have to do both (your method and spraying the shelving with mite spray)?

I generally put the media in the container first, microwave it , let it sit, then fill it with springtails by picking up small amounts of springs at a time.

I just wonder if there's a way to rid your cultures of mites forever?

 

Somehow they still get in no matter how careful I am. Does this mean we just have to do both (your method and spraying the shelving with mite spray)?

The food you feed them is also often a source of mites..

I just wonder if there's a way to rid your cultures of mites forever?

Unless you are fanatical about cleanliness then no..

Ed