I did this once successfully by dusting the flies heavily, and then letting them crawl out of the cup (tilted horizontally) as opposed to dumping them out. I repeated 2 or 3 times with fresh dust. I was told the dust would knock the mites off, and that the flies would crawl out much faster than the mites (head for the light).
Not very scientific, but my mite infested culture produced clean cultures that have been going for dozens of generations since.
Josh
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