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I use sterilite sweater boxes/32 quart I believe. I add 2 inches of bed-a-beast and a layer of magnolia leaves. I then add springtails. I sometimes add some coco-huts on occassion. I keep the cultures at room temp. and collecting from these cultures is very easy. I just sprinkle bakers yeast on top of the leaves and they swarm everywhere. I keep my cultures just moist enough to keep the bed-a-beast a darker color, but not sopping by no means. These are from the same tropical springtails that I bought a couple of years back. I have yet to need to purchase any extras. I literally have thousands in these springtails in each container. I use the coco-huts and leaves to seed tanks and just replace leaves and huts as needed.I am adding some pics of the cultures.